Melanoma cell sensitivity to docetaxal-induced apoptosis is determined by class III β-tubulin levels

Title: Melanoma cell sensitivity to docetaxal-induced apoptosis is determined by class III β-tubulin levels
Creator: Mhaidat, Nizar M.; Thorne, Rick F.; De Bock, Charles Edo; Zhang, Xu Dong; Hersey, Peter
Relation: FEBS Letters Vol. 582, Issue 2, p. 267-272
Publisher Link: http://dx.doi.org/10.1016/j.febslet.2007.12.014
Publisher: Elsevier
Resource Type: journal article
Date: 2008
Description: We have previously shown that Docetaxel-induced variable degrees of apoptosis in melanoma. In this report, we studied the β-tubulin repertoire of melanoma cell lines and show that class III β-tubulin expression correlated with Docetaxelresistance. Sensitive cells showed low levels of class III β-tubulin with little microtubular incorporation, whereas class III β-tubulin expression was higher in resistant cells and was incorporated into the cytoskeleton. As proof of concept, abrogation of class III by siRNA reverted Docetaxel-resistant cells to a sensitive phenotype, restoring the microtubular polymerisation response and promoting high levels of apoptosis through Bax activation. These results suggest that phenotypic expression of β-tubulin class III in melanoma may help identify patients with melanoma that can respond to taxanes.
Subject: melanoma; docetaxel; class III β-tubulin; apoptosis; taxanes
Identifier: http://hdl.handle.net/1959.13/43278
Identifier: uon:5398
Identifier: ISSN:0014-5793
Language: eng
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